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KMID : 0381920070370040219
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Korean Journal of Microscopy
2007 Volume.37 No. 4 p.219 ~ p.230
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Morphological Study of the Regeneration of the Mouse Olfactory Epithelial Cells after Destruction by Intranasal Zinc Sulfate Irrigation
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Kang Wha-Sun
Moon Young-Wha
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Abstract
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The morphological effects of intranasal zinc sulfate (5% solution) irrigation on the mouse olfactory epithelium and the regeneration process of olfactory receptor cells following nasal irrigation were studied with scanning and transmission electron microscope.
The results were as follows :
1. The septal epithelium except some basal cells was wholly detached from the basement membrane, during the first 6 to 24 hours after 5% zinc sulfate irrigation.
2. 3 days after ZnSO4 treatment, two layered septal epithelium was formed from basal cells. And microvilli were observed in the apical epithelium of newly formed olfactory epithelial cells.
3. 5 days after treatment, a lot of centrosomes and basal bodies were observed in the olfactory receptor cells, and cilia were lined up between microvilli on the apical membrane of olfactory receptor cells. And immature olfactory knob was first observed in the newly formed olfactory receptor cells. Mature olfactory knob was observed 1 week after treatment.
4. There are very many mature olfactory knobs in the olfactory receptor cells 2 weeks after intranasal zinc sulfate irrigation.
These results support that treatment with 5% zinc sulfate is a good experimental model for the regeneration of mammalian nervous tissues because this method could thoroughly detach the septal epithelium . During the regeneration of olfactory receptor cells, the surface membrane of the olfactory receptor cells widen the surface with the microvilli. Then cilia, which arranged in a line, substituted for the microvilli. The part of the surface membrane with cilia protruded and finally formed the olfactory vesicle.
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KEYWORD
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Regeneration, Olfactory receptor cell, Olfactory vesicle, Zinc sulfate
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